Table 3. Results of three ANOVAs testing the effects of ant origin, fungus origin, time since start of the experiment, directional antagonism from cultivars and Pseudonocardia, respectively, as well as the interaction between ant colony-of-origin and fungus colony-of-origin by time, on fungus weight (left) and actinobacterium coverage of ants (right).

	Fungus garden mass						Actinobacterium abundance
	Full duration of the experiment			From day 11 and onwards			From day 11–21
Effect	F	df	p	F	df	p	F	df	p
Ant origin	2.728	4	0.0281	1.587	4	0.1759	159.9	4	<0.0001
Fungus origin	63.23	8	<0.0001	66.94	8	<0.0001	19.85	8	<0.0001
Time	424.1	26	<0.0001	379.4	15	<0.0001	60.22	10	<0.0001
Ant origin * Time	1.128	104	0.1870	1.192	60	0.1576	3.062	40	<0.0001
Fungus origin * Time	6.465	208	<0.0001	5.732	120	<0.0001	1.103	80	0.2648
Inhibition of Pseudonocardia by cultivar	0.0586	1	0.8088	0.0788	1	0.7789	0.8391	1	0.3601
Inhibition of cultivar by Pseudonocardia	0.2028	1	0.6526	0.0913	1	0.7626	10.70	1	0.0011

Because actinobacterium abundance scores were only obtained from day 11 and onwards, and because of missing values, we performed the ANOVA on days 11–21 only.