Table 4. Results of ANOVAs on each of the seven individual combinational experiments listed in Table 2.
Fungus garden mass | Actinobacterium abundance | ||||||||
Full duration of the experiment | From day 11 and onwards | From day 11 and onwards | |||||||
Effect | F | df | p | F | Df | p | F | df | p |
Ants from colony A on fungus from colony A, K or D | |||||||||
Ant-fungus combination | 122.5 | 2 | <0.0001 | 96.01 | 2 | <0.0001 | 109.4 | 2 | <0.0001 |
Time | 83.07 | 15 | <0.0001 | 71.04 | 26 | <0.0001 | 6.035 | 10 | <0.0001 |
Ant-fungus combination*Time | 9.310 | 30 | <0.0001 | 8.883 | 52 | <0.0001 | 1.869 | 20 | 0.0304 |
Ants from colony C on fungus from colony C, J, or D | |||||||||
Ant-fungus combination | 4.106 | 2 | 0.0195 | 4.403 | 2 | 0.0137 | 31.58 | 2 | <0.0001 |
Time | 59.23 | 15 | <0.0001 | 70.57 | 26 | <0.0001 | 15.95 | 10 | <0.0001 |
Ant-fungus combination*Time | 1.364 | 30 | 0.1309 | 1.371 | 52 | 0.0706 | 1.479 | 20 | 0.1201 |
Ants from colony D on fungus from colony D, E, or J | |||||||||
Ant-fungus combination | 5.002 | 2 | 0.0086 | 7.221 | 2 | 0.0010 | 8.274 | 2 | 0.0006 |
Time | 45.85 | 15 | <0.0001 | 57.37 | 26 | <0.0001 | 7.483 | 10 | <0.0001 |
Ant-fungus combination*Time | 1.794 | 30 | 0.0174 | 1.793 | 52 | 0.0031 | 1.197 | 20 | 0.2866 |
Ants from colony C on fungus from colony C, I, or D | |||||||||
Ant-fungus combination | 4.151 | 2 | 0.0187 | 3.616 | 2 | 0.0291 | 11.02 | 2 | <0.0001 |
Time | 118.0 | 15 | <0.0001 | 135.6 | 26 | <0.0001 | 30.01 | 9 | <0.0001 |
Ant-fungus combination*Time | 1.079 | 30 | 0.3783 | 1.129 | 52 | 0.2802 | 0.946 | 18 | 0.5301 |
Ants from colony H on fungus from colony H, I, or K | |||||||||
Ant-fungus combination | 1.965 | 2 | 0.1457 | 3.992 | 2 | 0.0203 | 0.8979 | 2 | 0.4123 |
Time | 235.2 | 15 | <0.0001 | 247.7 | 26 | <0.0001 | 10.38 | 10 | <0.0001 |
Ant-fungus combination*Time | 0.8174 | 30 | 0.7306 | 0.7756 | 52 | 0.8556 | 0.4239 | 20 | 0.9826 |
Ants from colony A on fungus from colony A, I, or D | |||||||||
Ant-fungus combination | 153.2 | 2 | <0.0001 | 136.0 | 2 | <0.0001 | 7.125 | 2 | 0.0017 |
Time | 65.15 | 15 | <0.0001 | 67.32 | 26 | <0.0001 | 22.83 | 9 | <0.0001 |
Ant-fungus combination*Time | 9.602 | 30 | <0.0001 | 11.28 | 52 | <0.0001 | 1.470 | 18 | 0.1338 |
Ants from colony F on fungus from colony F, I, or D | |||||||||
Ant-fungus combination | 0.4526 | 2 | 0.6373 | 0.4943 | 2 | 0.6109 | 0.7743 | 2 | 0.4656 |
Time | 233.0 | 15 | <0.0001 | 239.8 | 26 | <0.0001 | 22.17 | 9 | <0.0001 |
Ant-fungus combination*Time | 0.6268 | 30 | 0.9270 | 0.6330 | 52 | 0.9715 | 0.4457 | 18 | 0.9702 |
The main factors ant-fungus pairing (nominal) and time (nominal) are included, in addition to the interaction between ant-fungus pair and time. Due to missing data as sub-colonies started to crash, the ANOVAs evaluating actinobacterium abundances could not be done over the entire duration of the experiment. Thus, for pairings A on fungus from colony A, K or D, C on fungus from colony C, J, or D, D on fungus from colony D, E, or J, and H on fungus from colony H, I, or K only days 11–23 were included. For the remaining pairings (C on fungus from colony C, I, or D; A on fungus from colony A, I, or D; and F on fungus from colony F, I, or D), tests were performed only on data from days 11–21.