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. 2010 Jan 15;5(1):e8748. doi: 10.1371/journal.pone.0008748

Table 4. Results of ANOVAs on each of the seven individual combinational experiments listed in Table 2.

Fungus garden mass Actinobacterium abundance
Full duration of the experiment From day 11 and onwards From day 11 and onwards
Effect F df p F Df p F df p
Ants from colony A on fungus from colony A, K or D
Ant-fungus combination 122.5 2 <0.0001 96.01 2 <0.0001 109.4 2 <0.0001
Time 83.07 15 <0.0001 71.04 26 <0.0001 6.035 10 <0.0001
Ant-fungus combination*Time 9.310 30 <0.0001 8.883 52 <0.0001 1.869 20 0.0304
Ants from colony C on fungus from colony C, J, or D
Ant-fungus combination 4.106 2 0.0195 4.403 2 0.0137 31.58 2 <0.0001
Time 59.23 15 <0.0001 70.57 26 <0.0001 15.95 10 <0.0001
Ant-fungus combination*Time 1.364 30 0.1309 1.371 52 0.0706 1.479 20 0.1201
Ants from colony D on fungus from colony D, E, or J
Ant-fungus combination 5.002 2 0.0086 7.221 2 0.0010 8.274 2 0.0006
Time 45.85 15 <0.0001 57.37 26 <0.0001 7.483 10 <0.0001
Ant-fungus combination*Time 1.794 30 0.0174 1.793 52 0.0031 1.197 20 0.2866
Ants from colony C on fungus from colony C, I, or D
Ant-fungus combination 4.151 2 0.0187 3.616 2 0.0291 11.02 2 <0.0001
Time 118.0 15 <0.0001 135.6 26 <0.0001 30.01 9 <0.0001
Ant-fungus combination*Time 1.079 30 0.3783 1.129 52 0.2802 0.946 18 0.5301
Ants from colony H on fungus from colony H, I, or K
Ant-fungus combination 1.965 2 0.1457 3.992 2 0.0203 0.8979 2 0.4123
Time 235.2 15 <0.0001 247.7 26 <0.0001 10.38 10 <0.0001
Ant-fungus combination*Time 0.8174 30 0.7306 0.7756 52 0.8556 0.4239 20 0.9826
Ants from colony A on fungus from colony A, I, or D
Ant-fungus combination 153.2 2 <0.0001 136.0 2 <0.0001 7.125 2 0.0017
Time 65.15 15 <0.0001 67.32 26 <0.0001 22.83 9 <0.0001
Ant-fungus combination*Time 9.602 30 <0.0001 11.28 52 <0.0001 1.470 18 0.1338
Ants from colony F on fungus from colony F, I, or D
Ant-fungus combination 0.4526 2 0.6373 0.4943 2 0.6109 0.7743 2 0.4656
Time 233.0 15 <0.0001 239.8 26 <0.0001 22.17 9 <0.0001
Ant-fungus combination*Time 0.6268 30 0.9270 0.6330 52 0.9715 0.4457 18 0.9702

The main factors ant-fungus pairing (nominal) and time (nominal) are included, in addition to the interaction between ant-fungus pair and time. Due to missing data as sub-colonies started to crash, the ANOVAs evaluating actinobacterium abundances could not be done over the entire duration of the experiment. Thus, for pairings A on fungus from colony A, K or D, C on fungus from colony C, J, or D, D on fungus from colony D, E, or J, and H on fungus from colony H, I, or K only days 11–23 were included. For the remaining pairings (C on fungus from colony C, I, or D; A on fungus from colony A, I, or D; and F on fungus from colony F, I, or D), tests were performed only on data from days 11–21.