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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Am J Transplant. 2009 Nov 24;10(1):162–167. doi: 10.1111/j.1600-6143.2009.02871.x

Figure 3. CD8+ memory T cell recall in the absence of IFN-I function.

Figure 3

(a) Spleen cells were harvested 90 days after transplanting wt or IFN-IR−/− 129S6 male skin onto wt or IFN-IR−/− 129S6 female recipients, respectively, and re-stimulated ex vivo with either male (allogeneic) or female (syngeneic) 129S6 splenocytes for 5 hrs to measure memory recall function. IFNγ and TNFα production were assessed by intracellular staining after gating on CD8+Uty+ cells. Values shown in allogeneic gates are percentages ± SD (n = 4 – 5, p > 0.05). (b) Second set rejection was measured > 4 weeks after first set rejection in either fully allogeneic (left panel, n = 6 – 8 mice/group) or H-Y mismatched (right panel, n = 9 – 10 mice/group) donor-recipient pairs. Accelerated second set rejection was observed in both wt and IFN-IR−/− groups.