Fig. 4.

Gel electrophoretic analysis of chemically modified Ocr using either ammonium hydroxide (N-series) or dimethylamine (D-series) as a nucleophile. Panels (a) and (b) show native 15% PAGE of the N- and D-series of chemically modified Ocr samples, respectively. Gels were run under non-denaturing reducing conditions. “WT” refers to the WT unmodified Ocr. The name given to each modified protein sample reflects the reaction time in minutes. (c) SDS-PAGE, using NuPAGE 4%–12% Bis–Tris gel, of the unmodified Ocr sample [same sample as shown in lanes WT in panels (a) and (b)]. (d) Unmodified Ocr analysed by blue native gel electrophoresis using the NativePAGE Novex® 4%–16% Bis–Tris gel system. The protein markers were supplied by Invitrogen. The Ocr sample was resolved into four discrete bands labelled a–d.