Fig. 5.

SDS-PAGE analysis of chemically modified Ocr using 15% acrylamide gel. (a) Analysis of the Ocr samples using dimethylamine as a nucleophile (D-series). The numbers for each sample correspond to the reaction time in minutes. (b) Analysis of the Ocr samples using ammonium hydroxide as a nucleophile (N-series). The numbers for each sample correspond to the reaction time in minutes. In both panels (a) and (b), the modified Ocr runs as two main bands: upper and lower bands corresponding to Ocr dimer and monomer, respectively. Note that the upper bands for the highly modified protein samples from the D- and N-series appear to resolve into two separate species (labelled a and b).