Table 1.
Summary of Crystallographic Data and Refinement
| 70S-RelE Complex Precleavage State |
70S-RelE Complex Postcleavage State |
Isolated RelE |
|
|---|---|---|---|
| Data Collection Statistics | |||
| Space group | P212121 | P212121 | P1211 |
| Unit cell dimensions | |||
| a, b, c, Å | a = 211.6, b = 452.0, c = 622.4 | a = 211.2, b = 451.4, c = 623.3 | a = 40.35, b = 60.79, c = 70.32 |
| α, β, γ | α = β = γ = 90 | α = β = γ = 90 | α = γ = 90, β = 104.53 |
| Resolution, Å | 50–3.3 (3.4–3.3) | 50–3.6 (3.7–3.6) | 19.5–2.5 (3.0–2.5) |
| Rsym, % | 16.3 (119.3) | 22.9 (114.1) | 9.6 (47.4) |
| I / σI | 9.80 (1.95) | 8.39 (2.11) | 11.8 (3.6) |
| Completeness, % | 99.8 (99.6) | 99.8 (99.7) | 89.4 (81.7) |
| Redundancy | 8.46 (7.43) | 8.47 (7.26) | 3.0 (2.4) |
| Refinement Statistics | |||
| Resolution, Å | 50.0–3.3 | 50.0–3.6 | 19.5–2.5 |
| Number of unique reflections | 883,810 | 681,993 | 11,259 |
| Rwork / Rfree, % | 21.9 / 24.7 | 21.5 / 24.8 | 22.6 / 26.8 |
| Molecules per a.s.u. | 2 | 2 | 3 |
| Number of atoms | |||
| RNA | 99,764 (per molecule) | 99,751 (per molecule) | − |
| Protein | 47,875 (per molecule) | 47,889 (per molecule) | 2,333 |
| Ions | 536 (per molecule) | 536 (per molecule) | − |
| Solvent | − | − | 18 |
| Average B factor | |||
| RNA | 113 | 106 | − |
| Protein | 123 | 117 | 67.9 |
| Rmsd from ideality | |||
| Bond lengths (Å) | 0.007 | 0.007 | 0.009 |
| Bond angles (°) | 1.17 | 1.19 | 1.36 |
X-ray data and refinement statistics. Numbers in parentheses indicate the values for the outermost resolution shell.