Figure 2. A: Amplification products from DNA extracted from formalin-fixed tissues. The 684-base pair products were obtained after 2 amplification steps involving 2 sets of primers. L — ladder DNA for size range; P — positive control (T. sergenti DNA); F6, F7, and F8 — DNA from spleens of 2 fetuses aborted at 6 and 7 mo of gestation and 1 calf born at 8 mo of gestation; C — DNA from positive dam; S — DNA from spleen of adult cow; J — positive-control DNA (from Japanese T. sergenti strain); N — DNA from normal tissue. B: Southern blot validation of PCR products in A.