Fig. 2.

Sucrose-induced currents and membrane depolarization in oocytes expressing OsSUT1 and OsSUT5. (A, B) In Na⁺, K⁺ and choline Ringer solutions at pH 5.6, SUT-expressing oocytes were voltage clamped at −40 mV and currents were recorded. Each trace represents a current induced by 10 mM sucrose in a modified Ringer solution. (C, D) Membrane potential was recorded using an oocyte impaled with a single electrode, referenced against the bath electrodes. Membrane depolarization was observed when the oocyte was perfused with Na Ringer solution at pH 5.6 containing sucrose at the indicated concentrations.