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. 2009 Nov 11;103(1):479–489. doi: 10.1152/jn.00835.2009

FIG. 2.

FIG. 2.

Quantitative analysis of phosphorylation of GluR1 on S831 and S845 in young and adult S831A and S845A mutants. A: no significant changes in the relative S845 phosphorylation or the total GluR1 levels in hippocampal samples obtained from young (3-wk-old) S831A mutants. Representative immunoblots are shown on the top panels. S831A mutant samples specifically lack signal when probed with S831 phosphospecific antibody (S831-p Ab). The relative S845 phosphorylation level (bottom left: S845 signal normalized to total GluR1 levels) and the total GluR1 level (bottom right) did not differ between WT and S831A. B: a significant reduction in the relative S845 phosphorylation (bottom left) and a significant increase in the total GluR1 level (bottom right) in hippocampal samples from adult (≥3-mo-old) S831A mutants. The observed decrease in the relative S845 phosphorylation level was largely due to the increase in the total GluR1 levels. *P < 0.001, t-test). Representative immunoblot images are shown in the top panel. S831A mutant samples specifically lack signal when probed with S831 phosphospecific antibody (S831-p Ab). C: no changes in the relative S831 phosphorylation (bottom left: S831 signal normalized to total GluR1 levels) and the total GluR1 levels (bottom right) in hippocampal samples of young (3-wk-old) S845A mutants. Representative immunoblot images are shown in the top panel. S845A mutant samples specifically lack signal when probed with S845 phosphospecific antibody (S845-p Ab). D: no significant changes in the relative S831 phosphorylation (bottom left) and GluR1 levels (bottom right) in hippocampal samples of adult (≥3-mo-old) S845A mutants. Representative immunoblot images are shown in the top panel. S845A mutant samples specifically lack signal when probed with S845 phosphospecific antibody (S845-p Ab).