Fig. 2.
Expression of recombinant BLV gp51/gp30T in expressed cells and supernatant. (A) Comparison of recombinant BLV gp51/gp30T- between expressed cells and supernatant by indirect enzyme-linked immunosorbent assay (I-ELISA) using reference positive (ST+) and negative (N) bovine sera. A: Hi-five cells only (infected), B: Supernatant from expressed cells, C: Control cells, D: Supernatant (control). The results were expressed as optical density (OD) value at 5 days post inoculation (DPI). (B) Time course profiles of recombinant BLV gp51/gp30T- secretion from the expressed Hi-five cells by I-ELISA using reference positive bovine serum (×25). The results were expressed as the OD value at 5 DPI.