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Analysis of the acid extracts of E. coli cells. The acid extracts of the wild-type, ΔcsdA, ΔsufS, ΔiscS, and ΔmoaD strains were analyzed using HPLC with a Capcell Pak C18 SG120 column after air oxidation. The volumes of the analyzed samples were 12.5 μl for the ΔmoaD strain and 25 μl for the other strains.
