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. 2009 Nov 23;285(4):2309–2317. doi: 10.1074/jbc.M109.046631

FIGURE 1.

FIGURE 1.

BCAR3 augments Cas-mediated enhancement of c-Src activity. A, co-overexpression of Cas and BCAR3 enhances Cas-dependent c-Src activity. COS-1 green monkey kidney cells were transfected with plasmids encoding wild type c-Src, FLAG-cortactin, pRK5 vector (V), Myc-Cas (C), and/or FLAG-BCAR3. FLAG immune complexes were generated from cell lysate 24 h post-transfection and immunoblotted with Tyr(P) and FLAG-specific antibodies (top two panels). Whole cell lysates were immunoblotted with Myc and FLAG antibodies (bottom two panels). IP, immunoprecipitation. B, BCAR3 requires Cas/c-Src binding to augment c-Src activity. COS-1 green monkey kidney cells were transfected with plasmids encoding wild type c-Src, FLAG-paxillin, pRK5 vector (V), Myc-Cas (C), Myc-Cas-TM (TM), and/or FLAG-BCAR3. Cells were lysed 24 h post-transfection, and proteins were analyzed as described above. Data shown are representative of 2–4 independent experiments.