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. 2009 Nov 23;285(4):2309–2317. doi: 10.1074/jbc.M109.046631

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Two distinct pools of Cas exist in BT549 cells. A, Cas and c-Src are present in a molecular complex. 400 μg of BT549 cell lysate were incubated with c-Src monoclonal antibody 2-17 (lane 2) or isotype-matched monoclonal antibody HA.11 as a control (lane 3). Immune complexes were collected and immunoblotted with the indicated antibodies. For comparison, 20 μg of cell lysate were immunoblotted (lane 1). The vertical separation indicates different exposure times between lysate and immunoprecipitation (IP) lanes. B, Cas and BCAR3 are present in a molecular complex. BCAR3 or control (preimmune serum) immune complexes were generated as described above from 350 μg of BT549 cell lysate, collected on protein A-agarose, and immunoblotted with the indicated antibodies (lanes 2 and 3). For comparison, 30 μg of cell lysate were immunoblotted (lane 1).