FIGURE 3.
TRβ and PGC-1α are associated with the rPDK4 gene promoter in vivo. ChIP assays were conducted on primary rat hepatocytes. Hepatocytes were treated with 100 nm T3 for 24 h prior to cross-linking as described under “Experimental Procedures.” A, model of the PDK4 gene and the location of the primers used for the ChIP assay is shown at the top of the figure. Antibodies to TRβ and PGC-1α or immunoglobulin G (IgG) were used for immunoprecipitation. The amplified PCR products using primers for the proximal, TRE, and upstream regions of the rPDK4 gene were resolved on an agarose gel. B, association of TRβ and PGC-1α with PDK4-TRE and -proximal were quantified using Quantity One software. These data are the average ± S.E. of four independent ChIP assays (**, p value 0.001 to 0.01).