FIGURE 5.

PGC-1α enhances the T₃ induction of rPDK4 gene. HepG2 hepatoma cells were transiently transfected with 2 μg of different rPDK4-luc constructs, 1 μg of pSV-PGC-1α or pSV, 1.0 μg of RSV-TRβ, and 0.1 μg of TK-Renilla. T₃ was added at a concentration of 100 nm for 24 h. All transfections were performed in duplicate and repeated three to six times. Luciferase assays were performed as described in the legend to Fig. 1. Results are expressed as fold induction by PGC-1α or T₃ compared with the untreated cells in each data set (*, p value 0.01 to 0.05; **, p value 0.001 to 0.01; ***, p value <0.001; Δ, p value 0.01 to 0.05 between −1256/+78 rPDK4 wild type and −1256/+78 rPDK4 −338/−370 ERRα mutant).