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Biochemistry of ER·SRC-RID complex formation. A, Bmax, Kd, and ERα availability measured for ERα interaction with SRC1, -2, or -3 RID in estradiol-treated cells (mean ± S.D., three independent experiments). B, the Bmax levels, which measure the concentration of ERα in an ER·SRC-RID complex, do not vary with ERα-CFP concentration in individual experiments (±S.E. for the curve fit within an experiment).
