FIGURE 2.

Cosmc restoration of activity of heat-denatured T-synthase is concentration dependent. A, purified HPC4-sT-syn (NT-syn) was heat-denatured in reconstitution buffer. Renaturation of the activity of the heat-denatured HPC4-sT-syn (DT-syn) was initiated by the addition of increasing concentrations of 6×His-sCosmc as indicated to DT-syn preparations, and percent T-synthase activity of each reaction was determined. B, time dependence of restoration of T-synthase activity from DT-syn by 6×His-sCosmc, in which reconstitution and assay of T-synthase was conducted at 37 °C, and the percent restored activity was determined at different time points as indicated. In A, each assay was performed in duplicate, two replicate experiments were performed, and data represent the average of all experiments. In B, each assay was performed in duplicate, three replicate experiments were performed, and data represent the average of all experiments. Error bars, ± 1 S.D. from the average.