FIGURE 2.

Ang II induces Akt-dependent PGC-1α dissociation from the catalase promoter. A, catalase promoter contains a conserved FoxO1-DBE: −1995TTATTTAC-1988. B, growth-arrested VSMCs were stimulated for the indicated times with 100 nm Ang II. C, VSMCs were pretreated with 10 μm LY294002, 10 μm PD98059, or 10 μm SB203580 for 30 min prior to treatment with 100 nm Ang II for 8 h. D, VSMCs were infected with Ad-HA-Akt(AAA) or Ad.Lac Z (control) and stimulated with Ang II for 8 h. ChIP assay was performed as described under “Experimental Procedures.” The result of one representative experiment is shown. The bar graphs represent averaged data (means ± S.E.) for PGC-1α binding to the catalase promoter (n = 3–5), expressed as fold change over basal. The relative ChIP units were calculated by the ratio of ChIP DNA to input DNA using densitometry. *, p < 0.05; **, p < 0.01 versus no Ang II treatment; ns, no significant difference.