FIGURE 5.

Ang II decreases endogenous catalase protein expression through Akt-dependent manner. A, growth-arrested VSMCs were stimulated with 100 nm Ang II for the indicated times. Protein expression was analyzed by Western blot with indicated antibodies. 48* and 72* denote that Ang II was added into medium again every 24 h. B–D, VSMCs were pretreated with 10 μm PD98059 (PD), LY294002 (LY), or SB203580 (SB, B) or with 1 μm Akt inhibitor V/triciribine (C) or infected with Ad.Akt-DN or Ad.Lac Z (control) (D) prior to stimulation with 100 nm Ang II for the indicated times. The result of one representative experiment is shown. The bar graphs represent the averaged data (means ± S.E.) for catalase expression (n = 3–5), expressed as fold change over basal. *, p < 0.05; **, p < 0.01; ns, no significant difference. DMSO, dimethyl sulfoxide.