FIGURE 5.

Aβ reduced the synaptic vesicular pool. A, electron micrographs show synapses from control (left) and 500 nm Aβ-treated (right) neurons. Rectangles illustrate the areas for quantification. Scale bars, 100 nm. B, columns showing the number of vesicles in both conditions from 12 selected active zones using a double-blind protocol (*, p < 0.05). C and D, effect of hypertonic sucrose solution (500 mOs) on miniature current frequencies in control and Aβ (500 nm)-treated neurons. Bars are mean ± S.E. from 18 neurons (***, p < 0.001; *, p < 0.05).