FIGURE 1.
Composition of Pax6 and No. 8 cDNAs and subdomains of their protein products. A, upper, canonical Pax6 cDNA is composed of 13 exons. Translation starts in exon 4 and ends in exon 13. lower, Pax6 protein contains the PD, linker region, HD, and PST domain. The boarders between the domains are marked by the numbers below. B, upper, the No. 8 cDNA spans the major part of exon 7, entire exons 8–11, and the unique exon 11α (filled black box) that is the intron between exons 11 and 12 in the Pax6 gene. Note that exon 11α contains a stop codon in fame with the preceding exons. Lower, the predicted protein product of No. 8 is shown. It includes the C-terminal end of the PD, linker region, HD, the N-terminal portion of the canonical PST domain (PSTN), and an S tail (filled black box) encoded by 11α. Gray indicates the regions that are present in full-length Pax6(S) cDNA and protein but are missing in No. 8.