Skip to main content
. 2009 Nov 23;285(4):2610–2621. doi: 10.1074/jbc.M109.071159

FIGURE 1.

FIGURE 1.

Cordycepin affects poly(A) tail length and cell proliferation at low concentrations. A, RNA ligation poly(A) tests on RNA from NIH3T3 cells treated with different doses of cordycepin. RNase H + Oligo(dT) indicates RNA from untreated cells digested with RNase H and oligo(dT) to remove the poly(A) tail as a control. The GenBank™ gene name abbreviations indicate which mRNAs were tested: Hif1a (hypoxia-inducible factor 1α), Cdkn1a (p21/Waf/Cip), Atf4 (activating transcription factor 4), Actg1 (gamma1 actin), Rps4X (X-linked ribosomal protein S4), and Rpl28 (ribosomal protein L28). B, Klenow priming poly(A) tests on a time course of cordycepin treatment. On the right side the panels shows the distribution of the intensity in each lane as a percentage of the maximum intensity in that lane on the vertical axis and the size of the poly(A) test products in base pairs on the horizontal axis. Black, oligo(dT) RNase A-treated sample; purple, no treatment; green, 15-min cordycepin; dark blue, 30-min cordycepin; light blue, 45-min cordycepin; and orange, 60-min cordycepin. C, Northern blot for Cdkn1a and β-actin (Act1b) on total RNA isolated from cells treated with 50 μm cordycepin for the indicated times. D, cell numbers after 72 h of treatment with cordycepin (medium refreshed daily, including drug).