FIGURE 4.

Identification of a GC-responsive region 1.3 kb upstream of the human DUSP1 start site. A genomic fragment extending from −1426 to −1096 with respect to the human DUSP1 TSS and various deletion derivatives of this fragment were subcloned into pGL3p. Responses to Dex were calculated as in Fig. 3C. The region −1366 to −1237 was identified as a minimal GC-responsive region and is hereafter known as GRR-1.3. Statistical comparisons are first against the parental vector pGL3p and second against the construct containing the minimal GC responsive region −1366 to −1237, which is hereafter referred to as pGL3p-GRR-1.3-Hs.