FIGURE 7.

Characterization of a GC-responsive region 29 kb upstream of the human DUSP1 start site. A, alignment of GBS-29 sequences of mouse, rat, human, and dog. This element was first described in the mouse DUSP1 locus (47); therefore, the mouse is regarded as the base sequence, and differences from it are highlighted. Matches to the consensus GNACANNNNG on the top or bottom strand are indicated by asterisks. B, dog, rat, mouse, or human GRR-29 fragments were cloned into pGL3p. pGL3p-GRR-29-Mm.-GBSm was created by mutagenizing both of the ACA triplets within the mouse GRR-29 fragment to GGG. pGL3p-GRR-29-Hs.>Mm. was generated by mutagenizing the sequence GAACATTCGG within human GRR-29 to the corresponding mouse sequence, GAATGTTCAG. This 3-nucleotide change creates a palindromic element with GNACANNNNG matches on both strands. Dex responses were calculated as in Fig. 3C. Differences from the parental vector pGL3p are shown to the right of each bar, and other statistical comparisons are as indicated. C, short oligonucleotides containing human and mouse GBS-29 were cloned into pGL4. Responses to Dex were calculated as in Fig. 3C. Differences from the parental vector pGL4 are shown to the right of each bar, and other statistical comparisons are as indicated.