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Kinetic parameters of wild type Xyn10B and mutant xylanases
| Enzyme | kcat | Km | kcat/Km |
|---|---|---|---|
| min−1 | m | min−1m−1 | |
| Xyn10B wild type | 7.80 × 103 | 2.834 × 10−3 | 3.27 × 106 |
| S15L | 6.93 × 104 | 1.820 × 10−3 | 3.81 × 107 |
| M93V | 7.06 × 104 | 1.712 × 10−3 | 4.13 × 107 |
| S15L/M93V | 5.48 × 104 | 1.497 × 10−3 | 3.66 × 107 |
| E137D/D323N | 9.64 × 104 | 2.495 × 10−3 | 3.86 × 107 |
