Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Nov 19;285(4):2796–2806. doi: 10.1074/jbc.M109.058461

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Expression and activities of cytoplasmically and luminally oriented FAAH-2 variants. A, constructs used in the study. ΔTMFAAH and ΔTMFAAH2 indicate the respective proteins lacking their putative N-terminal TM regions, N(TM) represents the N-terminal TM region of the indicated protein, CAL is the calreticulin signal sequence, and RFP represents red fluorescent protein. FLAG and Myc are the epitope tags used. B, relative expression of FAAH-FLAG, FAAH2-FLAG, and cytoplasmically and luminally facing FAAH and FAAH-2 variants. Transfected HeLa homogenates (20 μg) were separated by SDS-PAGE and probed with anti-FLAG and anti-β-actin antibodies. C, enzymatic activities of cell lysates containing the aforementioned proteins. Activity of CAL-ΔTMFAAH-FLAG was compared with FAAH-FLAG (black bars). For the rest of the proteins, activities were compared with FAAH-2 (blue bars). Inset: activities of FAAH-2 and its corresponding chimeras. **, p < 0.01 and ***, p < 0.001 (n = 4–6).