TABLE 3.
Residues of Synechocystis Slr0642 mutagenized in round 3
Strictly conserved residues are indicated by a plus sign, conservatively replaced residues by “c”, and non-conserved residues by a minus sign.
| Residue | Conservation pattern in proven folate carriers |
Critical | |||
|---|---|---|---|---|---|
| Slr0642 | At2g32040 | FT1 | FT5 | ||
| Leu-54 | + | + | − | − | No |
| Val-56 | + | + | − | − | No |
| Ser-57 | + | + | c | − | No |
| Phe-58 | + | + | c | − | No |
| Leu-60 | + | + | c | c | No |
| Ser-150 | + | + | − | − | No |
| Thr-259 | + | + | c | − | Yes |
| Ser-264 | + | + | c | + | No |
| Thr-271 | + | + | + | − | No |
| Asn-272 | + | + | − | − | No |
| Arg-335 | + | + | − | − | Yes |
| Met-384 | + | − | c | c | Yes |