Figure 7.

RARα ligands can inhibit estrogen–ER-mediated transcriptional activity. Hormone-depleted cells were treated with vehicle, estrogen, RA (ATRA), or estrogen + RA for 6 h, and ChIP was performed for ER (A), RARα (B), and p300 (C). Real-time PCR of a number of ER-binding regions was assessed. (D) Changes in mRNA levels of several regulated genes. (E) Western blot of total protein levels in treated cells. The data are the average of three independent experiments, ±SD. (*) P < 0.05; (**) P < 0.01.