Table 2.

Sample sizes needed to detect differences between transgene classes

t test	AdhS	Xdh	AdhF	{{AdhF}}
Difference in means = 10%, α = 0.05, 1 − β ≥ 0.8, n ≥
Unpaired
ρ = 0	291	133	35	14
Paired
ρ = 0.7	89	42	12	6
ρ = 0.8	60	29	9	5
ρ = 0.9	31	16	6	4
Difference in means = 20%, α = 0.05, 1 − β ≥ 0.8, n ≥
Unpaired
ρ = 0	75	35	11	6
Paired
ρ = 0.7	24	12	5	4
ρ = 0.8	13	9	4	3
ρ = 0.9	10	6	4	3

Sample sizes (n per class) required to detect (by t test) mean expression differences between transgene classes are calculated based on given true difference of 10% or 20%; type I error rate (α) of 0.05; power (1 − β) of at least 0.8; correlation with position (ρ) of 0, 0.7, 0.8, or 0.9; and coefficient of variation (SD/mean) among genomic positions. A range of typical coefficients of variation is represented by Adh^S (0.429), Xdh (0.288), Adh^F (0.145), and Adh^F flanked by binding sites for the su(Hw) protein (0.086), denoted as {{Adh^F}}.