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. 2009 Dec 30;8:60. doi: 10.1186/1476-511X-8-60

Table 1.

The primer Sequences Used for PCR Amplification

Gene Genebank
Accession No.
Annealing temperature Primer sequence 5' to 3'
Per2 NM_011066 58°C Forward Primer: CAGACTCATGATGACAGAGG
Reverse Primer: GAGATGTACAGGATCTTCCC

Bmal1 NM_007489 62°C Forward Primer: CACTGACTACCAAGAAAGTATG
Reverse Primer: ATCCATCTGCTGCCCTGAGA

Clock NM_007715 58°C Forward Primer: CTTCCTGGTAACGCGAGAAAG
Reverse Primer: TCGAATCTCACTAGCATCTGACT

Cry1 NM_007771 58°C Forward Primer: CACTGGTTCCGAAAGGGACTC
Reverse Primer: CTGAAGCAAAAATCGCCACCT

Rev-Erbα NM_145434 61°C Forward Primer: TACATTGGCTCTAGTGGCTCC
Reverse Primer: CAGTAGGTGATGGTGGGAAGTA

PPARα NM_011144 61°C Forward Primer: TCGGCGAACTATTCGGCTG
Reverse Primer: GCACTTGTGAAAACGGCAGT

RXRα NM_009024 61°C Forward Primer: CTGCACTCTCCTATCAGCACC
Reverse Primer: AGTCCCGAAGCCCAATGTG

GAPDH BC_083149 62°C Forward Primer: ACAGCCGCATCTTCTTGTGCAGTA
Reverse Primer: GGCCTTGACTGTGCCGTTGAATTT