Figure 5.

Relative abundance of different CPEB3 transcript patterns during development of the retina. The levels of each mRNA was normalized to the level of MAPK1 mRNA in the same sample. All the transcript variants demonstrated similar increasing trends during postnatal development. The relative abundance at any given age demonstrated that the "-69+24" pattern was the most abundant in exon 5-7 region; the "ex 11 + ex 12" pattern was the most abundant in the region surrounding exon 11; and "partial exon skipping within exon 4" pattern was abundant. For age P14, n = 3; for all the other ages, n = 4. Error bars represented standard error of the mean (SEM). The amplification efficiencies for all the primer sets used in realtime PCR were listed in the table below the graph.