Table 1. HLA-A24, -B7, -DR2, -DR3, and -DR4 restriction of 54 peptides of influenza proteins M1, NP, PA, PB1, and PB2 that contain conserved sequences of 9 or more amino acids.
| Protein | ELISpot positive 17 aa peptide* | A24# | B7 | DR2 | DR3 | DR4 |
| M1 | 169 TNPLIRHENRMVLASTT 185 | - | - | 56±5 (0.1) | 120±4 (0.1) | - |
| 175 HENRMVLASTTAKAMEQ 191 | - | - | - | - | 165±1 (0.1) | |
| 181 LASTTAKAMEQMAGSSE 197 | - | - | - | - | 115±21 (1) | |
| NP | 7 KRSYEQMETDGERQNAT 23 | - | - | - | - | 52±29 (0.1) |
| 31 RMIGGIGRFYIQMCTEL 47 | 45±5 (0.1) | - | - | - | - | |
| 37 GRFYIQMCTELKLNDYE 53 | - | - | - | 66±7 (1) | - | |
| 73 ERRNKYLEEHPSAGKDP 89 | - | - | - | - | 121±1 (0.1) | |
| 103 KWVRELVLYDKEEIRRI 119 | - | - | - | 614±21 (0.1) | - | |
| 109 VLYDKEEIRRIWRQANN 125 | - | - | - | 501±42 (0.1) | - | |
| 133 LTHIMIWHSNLNDTTYQ 149 | - | - | 238±59 (0.1) | - | - | |
| 402 SAGQISTQPTFSVQRNL 418 | - | - | 207±3 (0.1) | - | - | |
| 408 TQPTFSVQRNLPFDKTT 424 | - | - | 110±14 (1) | 41±2 (10) | - | |
| PA | 42 LEVCFMYSDFHFINEQG 58 | - | - | 64±11 (1) | - | - |
| 126 EVHIYYLEKANKIKSEK 142 | - | - | - | - | 37±11 (0.1) | |
| 132 LEKANKIKSEKTHIHIF 148 | - | - | - | - | 41±10 (0.1) | |
| 558 SRPMFLYVRTNGTSKIK 574 | - | - | - | - | 114±24 (0.1) | |
| PB1 | 31 SHGTGTGYTMDTVNRTH 47 | - | - | - | - | 106±1 (0.1) |
| 37 GYTMDTVNRTHQYSERG 53 | - | - | - | - | 125±11 (0.1) | |
| 120 DKLTQGRQTYDWTLNRN 136 | - | - | - | 142±6 (0.1) | - | |
| 126 RQTYDWTLNRNQPAATA 142 | - | - | - | 78±0 (0.1) | - | |
| 328 NQPEWFRNILSIAPIMF 344 | - | 60±8 (10) | - | - | - | |
| 340 APIMFSNKMARLGKGYM 356 | - | - | - | 175±0 (0.1) | - | |
| 352 GKGYMFESKSMKLRTQI 368 | - | - | 52±2 (1) | - | - | |
| 358 ESKSMKLRTQIPAEMLA 374 | - | - | 84±20 (0.1) | - | - | |
| 465 RFYRTCKLLGINMSKKK 481 | - | - | 231±73 (1) | - | - | |
| 471 KLLGINMSKKKSYINRT 487 | - | - | - | 116+10 (0.1) | - | |
| 489 TFEFTSFFYRYGFVANF 505 | 213±9 (0.1) | - | - | - | - | |
| 495 FFYRYGFVANFSMELPS 511 | 210±25 (0.1) | - | - | - | - | |
| 507 MELPSFGVSGVNESADM 523 | - | - | - | - | 274±15 (0.1) | |
| 519 ESADMSIGVTVIKNNMI 535 | - | - | 75±10 (0.1) | - | - | |
| 525 IGVTVIKNNMINNDLGP 541 | - | - | 159±53 (0.1) | - | - | |
| 537 NDLGPATAQMALQLFIK 553 | 92±2 (1) | - | - | - | - | |
| 548 LQLFIKDYRYTYRCHRG 564 | - | - | 61±2 (1) | 230+23 (0.1) | 97±30 (0.1) | |
| 554 DYRYTYRCHRGDTQIQT 570 | - | - | 109±13 (1) | 166±22 (0.1) | 76±2 (0.1) | |
| 560 RCHRGDTQIQTRRSFEI 576 | - | - | 194±47 (0.1) | - | - | |
| 650 GPAKNMEYDAVATTHSW 666 | - | - | - | 142±45 (0.1) | 41±9 (0.1) | |
| 656 EYDAVATTHSWVPKRNR 672 | - | - | - | - | 59±2 (0.1) | |
| 680 RGILEDEQMYQRCCNLF 696 | 78±4 (0.1) | - | - | 181±10 (0.1) | - | |
| PB2 | 42 NPSLRMKWMMAMKYPIT 58 | - | - | - | - | 166±3 (0.1) |
| 48 KWMMAMKYPITADKRIT 64 | - | - | - | - | 161±18 (0.1) | |
| 54 KYPITADKRITEMIPER 70 | - | - | - | 499±4 (0.1) | - | |
| 126 KHGTFGPVHFRNQVKIR 142 | - | - | - | - | 316±20 (0.1) | |
| 132 PVHFRNQVKIRRRVDIN 148 | - | - | - | - | 311±37 (0.1) | |
| 256 DQSLIIAARNIVRRAAV 272 | - | - | - | 169±12 (0.1) | - | |
| 369 RATAILRKATRRLIQLI 385 | - | - | - | - | 54±2 (0.1) | |
| 434 LLRHFQKDAKVLFLNWG 450 | - | - | - | 444±14 (0.1) | - | |
| 458 MGMIGILPDMTPSTEMS 474 | - | - | - | - | 238±5 (0.1) | |
| 464 LPDMTPSTEMSMRGVRV 480 | - | - | - | 324±28 (0.1) | - | |
| 500 RFLRVRDQRGNVLLSPE 516 | - | 184±3 (0.1) | - | - | - | |
| 524 TEKLTITYSSSMMWEIN 540 | - | - | 151±67 (0.1) | - | - | |
| 536 MWEINGPESVLINTYQW 552 | 289±16 (0.1) | - | - | - | - | |
| 542 PESVLINTYQWIIRNWE 558 | 226±5 (0.1) | - | - | - | - | |
| 548 NTYQWIIRNWETVKIQW 564 | 322±44 (0.1) | - | 96±9 (0.1) | - | - | |
| 630 RMQFSSLTVNVRGSGMR 646 | - | - | 104±16 (0.1) | - | - | |
| ELISpot responses | 8 | 2 | 16 | 17 | 20 |
*Conserved aa are boldface. Consecutive peptides overlapping by 11 aa are aligned.
#Numbers are representative average IFN-γ spots forming cells per million splenocytes of individual transgenic mice that were positive at 10 µg/ml of peptide concentration. Number (10, 1 or 0.1) in parenthesis represents the lowest concentration of peptide (µg/ml) giving positive ELISpot response in peptide titration.
-represents no positive IFN-γ ELISpot response.