Figure 1.

Cyclooxygenase-2 (Cox-2) inhibitors attenuate immunoglobulin M (IgM) and IgG production. Purified human B cells were left untreated or stimulated with oligodeoxynucleotide 2395 plus anti-IgM and cultured for 7 days in the presence of vehicle control (black), 5 μm, 10 μm or 20 μm SC-58125 (white) or NS-398 (grey). (a) IgM and (b) total IgG were measured in media supernatants by enzyme-linked immunosorbent assay. IgG1 (c), IgG2 (d), IgG3 (e) and IgG4 (f) were assessed using a Luminex multiplex assay. Peripheral blood mononuclear cells were cultured for 24 hr in the presence of SC-58125 or NS-398 following stimulation with CpG plus anti-IgM. (g) Prostaglandin (PG) E₂ was measured in supernatants, demonstrating that concentrations of selective inhibitors used abrogated Cox-2 activity. Data are represented as mean ± SD compared with vehicle alone, *P < 0·05.