Figure 6.

Effect of sodium pervanadate on the tyrosine phosphorylation of suppressor of cytokine signalling 3 (SOCS-3) in lipopolysaccharide (LPS)-stimulated macrophages from wild-type and tumour necrosis factor-α (TNF-α)-deficient mice. (a) Macrophages from wild-type mice were pre-treated with sodium pervanadate (5, 25 or 50 μm) for 1 hr and incubated with LPS (0·1 μg/ml) for 4 hr. (b) Macrophages from TNF-α-deficient mice were pre-treated with sodium pervanadate (50 μm) for 1 hr and incubated with LPS (0·1 μg/ml) or LPS plus TNF-α (15 ng/ml) for 4 hr. Macrophages from wild-type mice were treated with pervanadate, TNF-α or LPS. Cellular lysates were immunoprecipitated with anti-SOCS-3 antibody and the precipitates were analysed with immunoblotting with anti-phosphotyrosine or anti-SOCS-3 antibody. A typical experiment of three independent experiments is shown.