Figure 5.

(a) Neutrophil accumulation within the pulp chamber of infected animals was assessed by immunohistochemistry. Sections of mandibles 3 days after infection were reacted with rat anti-neutrophil antibody 7/4, and the length of the pulp chamber with neutrophil accumulation was measured; n = 4 or 5. (b) Neutrophil elastase (NE) was measured by quantitative polymerase chain reaction in RNA from infected mandibles 3 days after infection, and normalized to control genes. *P < 0·05, n = 6 or 7.