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. 2010 Jan;129(1):133–145. doi: 10.1111/j.1365-2567.2009.03129.x

Figure 2.

Figure 2

Figure 2

Comparative flow cytometric analysis of surface antigens on cells cultured for 7 days in granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 with or without stimulation with lipopolysaccharide (LPS) or CD40L. (a) Cells were gated based on side scatter/forward scatter (SSC/FSC) parameters. (b) Expression of surface antigens on non-stimulated cells (None), LPS-stimulated cells (LPS) or CD40L-stimulated cells (CD40L). Black lines, anti-chicken cell surface marker monoclonal antibodies (mAbs); grey lines, isotype control mAbs. (c) DEC-205 expression on live (Surface) or saponin-permeabilized (Intracellular) chicken bone marrow-derived dendritic cells (chBM-DCs). (d) Proportions of double-positive cells are shown in dot plots. The data shown are representative of three independent experiments.