Table 2.
Real-time quantitative reverse transcriptase–polymerase chain reaction (RT-PCR) probes and primers
| RNA target | Probe/primer sequence (5′-3′) | Accession number |
|---|---|---|
| 28S | Probe (FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA) | X59733 |
| F GGCGAAGCCAGAGGAAACT | ||
| R GACGACCGATTTGCACGTC | ||
| IFN-γ | Probe (FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA) | Y07922 |
| F GTGAAGAAGGTGAAAGATATCATGGA | ||
| R GCTTTGCGCTGGATTCTCA | ||
| IL-1β | Probe (FAM)-CCACACTGCAGCTGGAGGAAGCC-(TAMRA) | AJ245728 |
| F GCTCTACATGTCGTGTGTGATGAG | ||
| R TGTCGATGTCCCGCATGA | ||
| IL-2 | Probe (FAM)-ACTGAGACCCAGGAGTGCACCCAGC-(TAMRA) | AJ224516 |
| F TTGGAAAATATCAAGAACAAGATTCATC | ||
| R TCCCAGGTAACACTGCAGAGTTT | ||
| IL-4 | Probe (FAM)-AGCAGCACCTCCCTCAAGGCACC-(TAMRA) | AJ621735 |
| F AACATGCGTCAGCTCCTGAAT | ||
| R TCTGCTAGGAACTTCTCCATTGAA | ||
| IL-6 | Probe (FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(TAMRA) | AJ309540 |
| F GCTCGCCGGCTTCGA | ||
| R GGTAGGTCTGAAAGGCGAACAG | ||
| IL-12α | Probe (FAM)-CCAGCGTCCTCTGCTTCTGCACCTT-(TAMRA) | AY262751 |
| F TGGCCGCTGCAAACG | ||
| R ACCTCTTCAAGGGTGCACTCA | ||
| IL-13 | Probe (FAM)-CATTGCAAGGGACCTGCACTCCTCTG-(TAMRA) | AJ621735 |
| F CACCCAGGGCATCCAGAA | ||
| R TCCGATCCTTGAAAGCCACTT | ||
| CXCLi1 | Probe (FAM)-TCGCTGAACGTGCTTGAGCCATACCTT-(TAMRA) | Y14971 |
| F TGGCTCTTCTCCTGATCTCAATG | ||
| R GCACTGGCATCGGAGTTCA | ||
| CXCLi2 | Probe (FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-(TAMRA) | AJ009800 |
| F GCCCTCCTCCTGGTTTCAG | ||
| R TGGCACCGCAGCTCATT |
F, forward primer; IFN, interferon; IL, interleukin; R, reverse primer.