Table 2.

Clone, pretreatment for epitope desmasking, dilution and incubation times for primary antibodies

Antibody	Clone	Pretreatment	Dilution	Incubation time (min)
Vimentina	V9	Citrate 0.01 M; pH 6.0; 95°C; 30 min	1:800	40
α-Smooth muscle actina	1A4	No pretreatment	1:300	40
Desmina	DE-R-11	Citrate 0.01 M; pH 6.0; 95°C; 30 min	1:100	40

^aDAKO, Carpinteria, CA, USA