Figure 1. Clk2 is regulated by feeding/fasting and insulin.
(A) Clk2 protein is induced in the mouse liver by refeeding. Western Blot analysis of liver whole cell extracts from mice sacrificed at indicated time during a feeding-fasting-refeeding time course. (B) Clk2 mRNA levels are not regulated by fasting/feeding in the mouse liver. Q-RT-PCR analysis of Clk2, Pepck, and PGC-1α gene expression from total liver RNA isolated from mice in (A). Data presented as average +/- SEM, each bar N=2-3. (D) Clk2 protein is induced by insulin downstream of PI3K and Akt. Whole cell extracts from FAO hepatoma cells grown in RPMI + 0.5%BSA for 24 hours and treated with indicated inhibitor overnight prior to insulin stimulation for 1 hour.