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. 2009 Nov 4;29(44):13823–13836. doi: 10.1523/JNEUROSCI.3574-09.2009

Figure 8.

Figure 8.

Loss of nongenomic signaling and neuroprotective actions of E2 in the hippocampus after long-term E2 deprivation. A, NeuN (red) and Fluoro-Jade B (green) staining results in hippocampal CA1 of long-term E2-deprived animals treated with E2 or Pla. Note that E2 loses its ability to protect CA1 neurons against ischemic damage after long-term E2 deprivation. B, Effect of long-term E2 deprivation on nongenomic signaling by E2. Hippocampal CA1 protein lysate samples at 3 h reperfusion from Imm and 10W E2-replaced rats were analyzed by Western blot for p-Akt and Akt levels (a). b, Samples were immunoprecipitated with anti-ERα antibody and blotted with anti-p85 subunit of PI3K. Equal amount of protein was also Western blotted with anti-p85 to examine the total protein expression. *p < 0.05 versus Pla and 10W E2 groups. C, NeuN (red) and Fluoro-Jade B (green) staining reveals that EDC neuroprotection is lost in 10W animals. D, EDC loses the ability to activate Akt after 10 weeks of E2 deprivation. *p < 0.05 versus vehicle and 10 week EDC groups. n.s., No significant difference. E, In contrast to loss of E2 neural actions after long-term E2 deprivation, the trophic effect of E2 on the uterus is not lost after 10 weeks of E2 deprivation. Scale bars, 1 cm. Magnification, 1×.