Table 1.
Transmission of [PIN+] from Rnq1 mutants to wild-type Rnq1
| Frequency of [PIN+] transmission from rnq1 alleles expressed from: | ||||
| rnq1 alleles | RNQ1 promoter | ADH promoter | ||
| GFP foci | [PSI+] induction | GFP foci | [PSI+] induction | |
| empty vector | 0/10 | 0/10 | 0/5 | 0/5 |
| wild-type | 10/10 | 10/10 | 5/5 | 4/5 |
| S12P | 3/20 | 5/20 | 5/5 | 5/5 |
| V23A | 7/10 | 7/10 | 5/5 | 5/5 |
| L94A | 7/10 | 8/10 | 5/5 | 5/5 |
The plasmid-shuffled rnq1 strains were mated with [psi−] [pin−] wild-type RNQ1 cells; After confirming the presence of SDS-stable Rnq1 polymers in the selected diploids by SDD-AGE, the rnq1 mutant plasmids were segregated from these diploids; The [PIN+] state of these diploid colonies was examined upon transformation with Rnq1-GFP expressing plasmid or Sup35-NM domain overexpressing plasmid; Colonies showing Rnq1-GFP foci and [PSI+] inducibility were counted.