CsA inhibits adipocyte differentiation. (a) Undifferentiated 3T3-L1 cells. (b–d) T3L1 cells were differentiated for 6 days in the absence of control solvent (b), the presence of control solvent (c), or in CsA (10 μg/ml) (d) and stained with red oil O. The 3T3-L1 cells were differentiated in the presence of CsA (10 μg/ml) added at day 0 (d), day 1 (e), day 2 (f), day 3 (g), or day 4 (h).