Table 1.
Eukaryotic membrane proteins with high resolution structures.
| Protein | Expression Host | PDB coordinates | Resolution (Å) | Reference |
|---|---|---|---|---|
| TRANSMEMBRANE PROTEINS: BETA-BARREL | ||||
| Beta-Barrel Membrane Proteins: Mitochondrial Outer Membrane | ||||
| Human VDAC-1 voltage dependent anion channel | E. coli | 2K4T1, 2JK42 | 4 | [210,211] |
| Murine VDAC-1 voltage dependent anion channel | E. coli | 3EMN | 2.3 | [212] |
| TRANSMEMBRANE PROTEINS: ALPHA-HELICAL | ||||
| G Protein-Coupled Receptors | ||||
| Rhodopsin: Bovine Rod Outer Segment mutant N2C/D282C | COS cells | 2J4Y | 3.4 | [122] |
| Engineered turkey β1 adrenergic receptor | Trichoplusia ni | 2VT4 | 2.7 | [1] |
| Human β2 adrenergic receptor, from β2AR365-Fab5 (2R4R) and β2AR24/365- Fab5 complexes (2R4S) | Spodoptera frugiperda | 2R4R, 2R4S | 3.4/3.7 | [2] |
| Engineered human β2 adrenergic receptor | Spodoptera frugiperda | 2RH1, 3D4S | 2.4/2.8 | [3,4] |
| Human A2A adenosine receptor, In complex with a high-affinity subtype-selective antagonist ZM241385. | Spodoptera frugiperda | 3EML | 2.6 | [5] |
| Integrin Adhesion Receptors | ||||
| Human Integrin αIIbβ3 transmembrane-cytoplasmic heterodimer, NMR Structure | E. coli | 2KNC | [213] | |
| Snare Protein Family | ||||
| Syntaxin 1A/SNAP-25/Synaptobrevin-2 Complex from Rattus rattus | E. coli | 3HD7 | 3.4 | [214] |
| Ion Channels | ||||
| Kir3.1-Prokaryotic Kir Chimera: Mus musculus & Burkholderia xenovorans | E. coli | 2QKS | 2.2 | [7] |
| ASIC1 Acid-Sensing Ion Channel: Gallus gallus; 2QTS: N- and C-terminal deletions, 3HGC: minimal functional channel | Spodoptera frugiperda | 2QTS, 3HGC | 1.9/3.0 | [8,215] |
| ATP-gated P2X4 ion channel (apo protein): Danio rerio (zebra fish) (expressed in SF9 cells), 3.1 Å; Closed state. A construct, 3.5 Å: 3I5D | Spodoptera frugiperda | 3H9V, 3I5D | 3.1/3.5 | [216] |
| Kv1.2 Voltage-gated potassium Channel: Rattus norvegicus | Pichia pastoris | 2A79 | 2.9 | [9] |
| Kv1.2/Kv2.1 Voltage-gated potassium channel chimera: Rattus norvegicus | Pichia pastoris | 2R9R | 2.4 | [10] |
| Other Channels: Aquaporins and Glyceroporins | ||||
| Rat AQP4 aquaporin water channel, S180D mutant (2ZZ9) | Spodoptera frugiperda | 2D57, 2ZZ9 | 3.2/2.8 | [11,12] |
| Human AQP4 aquaporin water channel | Pichia pastoris | 3GD8 | 1.8 | [13] |
| Human AQP5 aquaporin water channel | Pichia pastoris | 3D9S | 2.0 | [14] |
| Plant SoPIP2;1 aquaporin: Spinacia oleracea in closed (1Z98) and open conformation (2B5F) | Pichia pastoris | 1Z98, 2B5F | 2.1/3.9 | [15] |
| Pichia pastoris AQY1 aquaporin, pH 3.5 (2W2E) and 8.0 (2W1P) | Pichia pastoris | 2W2E, 2W1P | 1.15/1.40 | [16] |
| Other Channels: Gap Junctions | ||||
| Human Connexin 26 (Cx26; GJB2) gap junction | Spodoptera frugiperda | 2ZW3 | 3.5 | [217] |
| Membrane-Associated Proteins in Eicosanoid and Glutathione Metabolism | ||||
| Human Microsomal Prostaglandin E Synthase 1: Human (Electron Diffraction) In complex with glutathione. | E. coli | 3DWW | 3.5 | [218] |
| Human 5-Lipoxygenase-Activating Protein (FLAP) with Bound MK-591 Inhibitor (2Q7M), FLAP with iodinated MK-591 analog (2Q7R) | E. coli | 2Q7M, 2Q7R | 4.0 | [219] |
| Human Leukotriene LTC4 Synthase in complex with glutathione | S. pombe | 2PNO | 3.3 | [220] |
| Human Leukotriene LTC4 Synthase in complex with glutathione/apo form | Pichia pastoris | 2UUH, 2UUI | 2.15/2.0 | [67] |
| ATP Binding Cassette Transporters | ||||
| Mus musculus P-Glycoprotein (3G5U), With bound QZ59-RRR (3G60) and QZ59-SSS (3G61) | Pichia pastoris | 3G5U, 3G60, 3G61 | 3.8/4.4/4.35 | [6] |
| P-type ATPase | ||||
| Human Na,K-ATPase Regulatory Protein FXYD1 | E. coli | 2JO11 | [221] | |
| Phospholamban homopentamer: Human sarcoplasmic reticulum | E. coli | 1ZLL1, 1FJK1, 1FJP1 | [222] | |
| Plasma Membrane H+-ATPase: Arabidopsis thaliana | S. cerevisiae | 3B8C | 3.6 | [223] |
1 Structure determined by NMR spectroscopy,
2 Structure determined by combining X-ray and NMR data.