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. 2009 Dec 18;7:109. doi: 10.1186/1479-5876-7-109

Figure 8.

Figure 8

Induction of antigen-specific CD8+ T cell responses by mRNA-electroporated mature IL-15 DCs. Short-term cultured IL-15 DCs were matured with our TLR7/8 agonist-based maturation cocktail (TLR-mDC), electroporated with mRNA encoding the influenza virus matrix protein M1 and cocultured with autologous PBLs for 6 days. (a) The expansion of M1-tetramer binding CD8+ T cells was determined by flow cytometry. The lower dot plot represents the observed percentage of M1-tetramer+ CD8+ T cells in one representative donor (n = 4; mean ± SEM percentage of M1-tetramer+ CD8+ T cells: 4.4 ± 2.9). Correct positioning of the M1-tetramer+ CD8+ gate was defined by the respective negative control, as exemplified in the upper dot plot. (b) Simultaneously, a fraction of the PBL was harvested and stimulated with an irrelevant HLA-A*0201-restricted peptide (CEA) or rechallenged with the immunodominant influenza matrix protein (M1). The mean ± SEM percentage of antigen-specific IFN-γ+ CD8+ T cells was determined by ICS, as specified in the "Methods" section (n = 4; *, P = 0.03).