Table 1.
Characteristics of the IBS-D and HC libraries.
| Library characteristic a | IBS-D library | HC libraryb | Combined libraries |
|---|---|---|---|
| No. of subjects in pooled sample | 23 | 10 | - |
| No. of sequences | 3267 | 3199 | 6466 |
| Average sequence %G+C | 55.7 | 56.2 | 55.9 |
| No. of OTUs (98%) c | 302 | 428 | 578 |
| Singletons | 131 | 170 | 245 |
| Coverage d | 96.0 | 96.2 | 94.7 |
| Shannon H e | 4.463 | 5.042 | 5.013 |
| Simpson 1/D f | 37.21 | 81.03 | 63.10 |
| Chao g | 515 | 613 | 899 |
| ACE h | 533 | 659 | 962 |
a. The library characteristics are based on separate and common alignments created for IBS-D and HC sequences.
b. Sequence data from Krogius-Kurikka et al. [16].
c. A 98% cut-off level for sequence similarity was used to delimit OTUs in the analyses.
d. Library coverage according to Good et al. [41].
e. Index for diversity [38].
f. Index for diversity [37].
g. Chao-1 richness estimator by Chao [39].
h. Abundance-based coverage estimator by Chao et al. [40].