Figure 3.

Direct binding of anti-β2GPI to recombinant β2GPI and DMs. Different concentrations of recombinant β2GPI and DMs were immobilized on nickel chelate microtiter wells. A constant amount of antibody was added, incubated, and washed, and the amount of antibody bound was detected by using an alkaline phosphatase conjugated second antibody. In A are results obtained with rabbit anti-β2GPI. In B are results obtained with anti-β2GPI 6203. Solid line, inhibitors that contain domain 1; broken lines, inhibitors that do not contain domain 1.