Table 4.
Direct binding of affinity-plurified aCL to nickel-chelated wells charged with recombinant deletion-mutant B2GP1 protein
| Antibody no. | 12345* | 1- - - - | 123- - | 1234- | -2345 | - -345 | - - -45 | - - - -5 |
|---|---|---|---|---|---|---|---|---|
| 6501 | 1.772† | 0.911 | 0.909 | 0.628 | 0.018 | 0.030 | 0.086 | 0.004 |
| 6626 | 1.527 | 0.560 | 1.250 | 0.563 | 0.008 | 0.022 | 0.086 | 0.028 |
| 6652 | 0.640 | 0.262 | 0.320 | 0.135 | 0.008 | 0.016 | 0.013 | 0.012 |
| 6632 | 1.419 | 0.351 | 0.121 | 0.003 | 0.031 | 0.004 | 0.000 | 0.013 |
| 7008 | 1.380 | 0.195 | 0.360 | 0.149 | 0.019 | 0.018 | 0.030 | 0.007 |
| 6701 | 0.948 | 0.388 | 0.841 | 0.715 | 0.002 | 0.002 | 0.000 | 0.000 |
| 6203 | 1.270 | 1.029 | 0.938 | 0.668 | 0.074 | 0.072 | 0.142 | 0.044 |
| 6641 | 2.555 | 0.252 | 0.530 | 0.145 | 0.045 | 0.019 | 0.112 | 0.018 |
| 6644 | 1.848 | 0.493 | 1.020 | 0.768 | 0.041 | 0.048 | 0.151 | 0.017 |
| 7101 | 1.257 | 0.804 | 0.951 | 0.843 | 0.056 | 0.062 | 0.142 | 0.059 |
| 7015 | 1.864 | 1.102 | 1.160 | 0.454 | 0.114 | 0.042 | 0.167 | 0.078 |
| Rabbit anti-B2 | 2.065 | 1.9737 | 1.971 | 1.708 | 1.873 | 1.933 | 1.941 | 1.663 |
Domains included in constructs.
Maximum OD for each recombinant deletion-mutant:antibody combination.