Table I.
Listed are the 15 most abundant lipid species, which were monitored by LC/MRM analysis, in negative ion mode. MRM transitions used for identification are listed in column two, and percent contribution of each species to total PE phospholipids is listed in column three. All identifications were confirmed using both positive and negative ion collision induced dissociation experiments. Results are expressed as mean ±SEM (n=3).
PE Lipid Species | MRM transistion used (m/z) |
Mole % Total PE phospholipids |
---|---|---|
16:0p/16:1 | 672→253 | 1.9 |
16:0a/16:1 | 688→253 | 5.9 |
16:0p/18:1 | 700→281 | 2.5 |
16:0a/18:2 | 714→279 | 20.4 |
16:0a/18:1 | 716→281 | 12.4 |
16:0p/20:4 | 722→303 | 23.8 |
16:0a/20:4 | 740→303 | 1.8 |
18:0a/18:1 | 744→281 | 1.4 |
16:0p/22:6 | 746→327 | 1.7 |
16:0p/22:5 | 748→329 | 2.3 |
16:0e/22:6 | 748→327 | 1.3 |
16:0p/22:4 | 750→331 | 2.0 |
18:0p/20:4 | 750→303 | 4.7 |
16:0a/22:6 | 762→327 | 10.5 |
18:0a/20:4 | 766→303 | 2.6 |