Figure 4.

Michaelis-Menten Kinetics of Recombinant Arabidopsis FC Lyase Expressed in Sf9 Cells.

FC lyase reactions were carried out as described in the Methods section. The standard error of the mean is indicated in (A)–(D); however, the errors bars are obscured by the plot symbols in (C) and (D).

(A) Linearity of FC lyase activity with time. Reactions contained 5 μg of protein and 5 μM [1-³H]FC.

(B) Linearity of FC lyase activity with protein. Reactions contained 4 μM [1-³H]FC and were incubated for 1 h.

(C) Substrate saturation kinetics of the FC lyase reaction. Reactions contained 3 μg of protein and were incubated for 1 h.

(D) Lineweaver-Burke plot of FC lyase substrate saturation kinetics. The data in Figure 4C were used for Lineweaver-Burke analysis.