Table 2.
Product | Production, pmol min−1 mg−1*** |
Farnesal + farnesol* | 1,333 ± 0 |
H2O2** | 1,195 ± 28 |
* NADase pretreatments were not performed. Consequently, farnesal and farnesol were resolved and quantified by gas chromatography and the amounts summed. Because this experiment was performed with a different preparation of recombinant Arabidopsis FC lyase (i.e. a different baculovirus infection) from that described in Figure 4, the activity is higher. ** Peroxide formation was slightly less than farnesal and farnesol, presumably due to peroxidase activity in Sf9 cell extracts. *** The standard error of the mean is indicated.